Inova Health System View Institution's Website 3 articles published in JoVE Cancer Research Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment Dave Mitchell*1,2, Allison L. Hunt*1,4, Kelly A. Conrads1,2, Brian L. Hood1,2, Sasha C. Makohon-Moore1,2, Christine Rojas1, G. Larry Maxwell1,3,4, Nicholas W. Bateman1,2,3, Thomas P. Conrads1,3,4 1Women’s Health Integrated Research Center, Gynecologic Cancer Center of Excellence, Department of Obstetrics and Gynecology, Uniformed Services University and Walter Reed National Military Medical Center, 2The Henry M. Jackson Foundation for the Advancement of Military Medicine, 3The John P. Murtha Cancer Center Research Program, Department of Surgery, Uniformed Services University, 4Women’s Health Integrated Research Center, Inova Women’s Service Line, Inova Health System This protocol describes a high-throughput workflow for artificial intelligence-driven segmentation of pathology-confirmed regions of interest from stained, thin tissue section images for enrichment of histology-resolved cell populations using laser microdissection. This strategy includes a novel algorithm enabling the transfer of demarcations denoting cell populations of interest directly to laser microscopes. Medicine A Human Glioblastoma Organotypic Slice Culture Model for Study of Tumor Cell Migration and Patient-specific Effects of Anti-Invasive Drugs Jonathon J. Parker1, Marcela Lizarraga2, Allen Waziri2, Kara M. Foshay2 1Department of Neurosurgery, Stanford University Hospital, Stanford University School of Medicine, 2Inova Neuroscience Institute Current ex vivo models of glioblastoma (GBM) are not optimized for physiologically relevant study of human tumor invasion. Here, we present a protocol for generation and maintenance of organotypic slice cultures from fresh human GBM tissue. A description of time-lapse microscopy and quantitative cell migration analysis techniques is provided. Biology Do-It-Yourself Device for Recovery of Cryopreserved Samples Accidentally Dropped into Cryogenic Storage Tanks Rohini Mehta1,2, Ancha Baranova1,2,3, Aybike Birerdinc1,2 1Molecular and Microbiology Department and Center for the Study of Genomics in Liver Diseases, George Mason University, 2Translational Research Institute, Inova Health System, 3Research Center for Medical Genetics RAMS Here we present a low cost, durable cryotolerant device for sample retrieval from Dewar tanks filled with liquid nitrogen. The ease of construction and modular design of the device makes the process of sample retrieval from cryogenic tanks safe and easy.