Pitzer College 2 articles published in JoVE Biology Design and Building of a Customizable, Single-Objective, Light-Sheet Fluorescence Microscope for the Visualization of Cytoskeleton Networks Nathan Felcher1, Daisy Achiriloaie1, Brian Lee2, Ryan McGorty3, Janet Sheung1,2,4 1W.M. Keck Science Department, Scripps College, 2W.M. Keck Science Department, Claremont McKenna College, 3Department of Physics and Biophysics, University of San Diego, 4W.M. Keck Science Department, Pitzer College This protocol describes in detail how to build a single-objective, light-sheet fluorescence microscope and its usage for visualizing cytoskeleton networks. Biology Analysis of Motility Patterns of Stentor During and After Oral Apparatus Regeneration Using Cell Tracking Janet Y. Sheung1, Megan Otsuka2, Gabriella Seifert3, Athena Lin4, Wallace F. Marshall4 1W. M. Keck Science Department, Scripps, Pitzer, and Claremont McKenna of The Claremont Colleges, 2W. M. Keck Science Department, Pitzer College, 3W. M. Keck Science Department, Scripps College, 4Department of Biochemistry and Biophysics, School of Medicine, University of California at San Francisco We present a protocol for the characterization of motility and behavior of a population of hundred micron- to millimeter-sized cells using brightfield microscopy and cell tracking. This assay reveals that Stentor coeruleus transitions through four behaviorally distinct phases when regenerating a lost oral apparatus.