Auburn University and University of Alabama Birmingham 1 article published in JoVE Behavior Training Dogs for Awake, Unrestrained Functional Magnetic Resonance Imaging Lily R. Strassberg*1, L. Paul Waggoner*2, Gopikrishna Deshpande*1,3,4,5, Jeffrey S. Katz*1,3,4,5 1Department of Psychology, Auburn University, 2Canine Performance Sciences Program, College of Veterinary Medicine, Auburn University, 3AU MRI Research Center, Department of Electrical & Computer Engineering, Auburn University, 4Alabama Advanced Imaging Consortium, 5Center for Neuroscience, Auburn University Magnetic resonance imaging (MRI) on unrestrained awake dogs is a new method with several advantages over imaging with physical or chemical restraint. This protocol introduces a cost-effective training method that minimizes training in the MRI environment, which can be expensive, and maximizes the subject pool available for canine functional MRI.