NETRI 1 article published in JoVE Cancer Research Co-culture of Glutamatergic Neurons and Pediatric High-Grade Glioma Cells Into Microfluidic Devices to Assess Electrical Interactions Quentin Fuchs1, Aurélie Batut2, Mélanie Gleyzes2, Jessica Rontard2, Louise Miny2, Margot Libralato2, Janaina Vieira2, Delphine Debis2, Florian Larramendy2, Thibault Honegger2, Melissa Messe1, Marina Pierrevelcin1, Benoit Lhermitte1,3, Monique Dontenwill1, Natacha Entz-Werlé1,4 1Team Tumoral signaling and therapeutic targets, UMR CNRS 7021 - Laboratory of Bioimaging and Pathologies, 2NETRI, 3Centre de Ressources Biologiques, Pathology department, University Hospital of Strasbourg, 4Pediatric Oncohematology unit, University Hospital of Strasbourg Recent works uncover the neuronal impact on high-grade pediatric glioma (pHGG) cells and their reciprocal interactions. The present work shows the development of an in vitro model co-culturing pHGG cells and glutamatergic neurons and recorded their electrophysiological interactions to mimic those interactivities.