Henan Agricultural University 1 article published in JoVE Biology Evaluation of Lipid Droplet Size and Fusion in Bovine Hepatic Cells Jingna Yang1, Fangyuan Kang1, Anqi Wei1, Wenyan Lu1, Xinfu Zhang1, Liqiang Han1 1College of Veterinary Medicine, Henan Agricultural University The present protocol describes how to use oil red O to dye lipid droplets (LDs), calculate the size and number of LDs in a fatty acid-induced fatty hepatocyte model, and use BODIPY 493/503 to observe the process of small LDs fusing into large LDs by live cell imaging.