Nagoya University Graduate School of Medicine 7 articles published in JoVE Neuroscience Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy Daisuke Kato1,2, Xiangyu Quan3, Yuta Tanisumi1,2, Zhongtian Guo1,2, Mitsuhiro Morita4, Tetsuya Takiguchi5, Osamu Matoba6, Hiroaki Wake1,2,6 1Department of Anatomy and Molecular Cell Biology, Nagoya University Graduate School of Medicine, 2Division of Multicellular Circuit Dynamics, National Institute for Physiological Sciences, National Institutes of Natural Sciences, 3Department of System Science, Kobe University Graduate School of System Informatics, 4Department of Biology, Graduate School of Sciences, Kobe University, 5Department of Information Science, Kobe University Graduate School of System Informatics, 6Center of Optical Scattering Image Science, Kobe University We developed a two-photon holographic microscope that can visualize, assess, and manipulate neural activity using high spatiotemporal resolution, with the aim of elucidating the pathogenesis of neuropsychiatric disorders that are associated with abnormal neural activity. Cancer Research Portal Vein Injection of Colorectal Cancer Organoids to Study the Liver Metastasis Stroma Hiroki Kobayashi1,2,3,4, Krystyna A. Gieniec1,2, Jia Q. Ng1,2, Jarrad Goyne1,2, Tamsin R. M. Lannagan1,2, Elaine M. Thomas1,2, Georgette Radford1,2, Tongtong Wang1,2, Nobumi Suzuki1,2,5, Mari Ichinose1,2, Josephine A. Wright2, Laura Vrbanac1,2, Alastair D. Burt6, Masahide Takahashi3,4,7, Atsushi Enomoto3, Daniel L. Worthley2, Susan L. Woods1,2 1Adelaide Medical School, University of Adelaide, 2South Australian Health and Medical Research Institute (SAHMRI), 3Department of Pathology, Nagoya University Graduate School of Medicine, 4Division of Molecular Pathology, Center for Neurological Disease and Cancer, Nagoya University Graduate School of Medicine, 5Department of Gastroenterology, Graduate School of Medicine, The University of Tokyo, 6Translational and Clinical Research Institute, Newcastle University, 7International Center for Cell and Gene Therapy, Fujita Health University Portal vein injection of colorectal cancer (CRC) organoids generates stroma-rich liver metastasis. This mouse model of CRC hepatic metastasis represents a useful tool to study tumor-stroma interactions and develop novel stroma-directed therapeutics such as adeno-associated virus-mediated gene therapies. Medicine Near Infrared Photoimmunotherapy for Mouse Models of Pleural Dissemination Hirotoshi Yasui1, Yuko Nishinaga1, Shunichi Taki1, Kazuomi Takahashi1, Yoshitaka Isobe1, Kazuhide Sato1,2,3 1Respiratory Medicine, Nagoya University Graduate School of Medicine, 2Nagoya University Institute for Advanced Research, 3B3-Unit, Advanced Analytical and Diagnostic Imaging Center (AADIC)/Medical Engineering Unit (MEU), Nagoya University Institute for Advanced Research Near-infrared photoimmunotherapy (NIR-PIT) is an emerging cancer therapeutic strategy that utilizes an antibody-photoabsorber (IR700Dye) conjugate and NIR light to destroy cancer cells. Here, we present a method to evaluate the antitumor effect of NIR-PIT in a mouse model of pleural disseminated lung cancer and malignant pleural mesothelioma using bioluminescence imaging. Developmental Biology A Mouse Distraction Osteogenesis Model Masahito Fujio1, Yusuke Osawa2, Masaki Matsushita2, Kota Ogisu1, Shuhei Tsuchiya1, Hiroshi Kitoh2, Hideharu Hibi1 1Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, 2Department of Orthopaedic Surgery, Nagoya University Graduate School of Medicine We present a mouse tibial distraction osteogenesis model developed using a custom-made distractor. The use of a mouse as an analysis target is advantageous for advancing research. Developmental Biology Ovarian Tissue Culture to Visualize Phenomena in Mouse Ovary Kouji Komatsu1, Akira Iwase2,3, Tomohiko Murase2, Satoru Masubuchi1 1Department of Physiology, Aichi Medical University, 2Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 3Department of Maternal and Perinatal Medicine, Nagoya University Hospital Ovarian tissue cultures can be used as models of follicle development, ovulation, and follicle atresia and indicate regulatory mechanisms of dynamic ovarian processes. Biology Use of Anti-phospho-girdin Antibodies to Visualize Intestinal Tuft Cells in Free-Floating Mouse Jejunum Cryosections Yuka Mizutani1, Daisuke Kuga2, Machiko Iida1, Kaori Ushida3, Tsuyoshi Takagi1, Yoshihito Tokita1, Masahide Takahashi3, Masato Asai1,3 1Division of Perinatology, Institute for Developmental Research, Aichi Human Service Center, 2Surgery Department, Anjo Kosei Hospital, 3Department of Pathology, Nagoya University Graduate School of Medicine Kuga et al. discovered that phosphorylation-status specific antibodies against the actin binding protein girdin phosphorylated at tyrosine 1798 (pY1798) can be used to label tuft cells (TCs). This protocol allows robust visualization of TCs using immunofluorescent staining of free-floating jejunum cryosections with pY1798 antibodies. Cancer Research Murine Experimental Model of Original Tumor Development and Peritoneal Metastasis via Orthotopic Inoculation with Ovarian Carcinoma Cells Yoshihiro Koya1,2, Hiroaki Kajiyama2, Wenting Liu1,2, Kiyosumi Shibata2, Takeshi Senga3, Fumitaka Kikkawa2 1Bell Research Center for Reproductive Health and Cancer, 2Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 3Department of Cancer Biology, Nagoya University Graduate School of Medicine To clarify the multistep process of peritoneal dissemination of ovarian carcinoma, the current study presents a murine experimental model of original tumor development and peritoneal metastasis via orthotopic inoculation with these tumor cells.