Heilongjiang Academy of Medical Science 1 article published in JoVE Biology Detecting Establishment of Shared Blood Supply in Parabiotic Mice by Caudal Vein Glucose Injection Xin Liu1, Xue Bai1, Mingqi Li1, Huimin Li1, Yong Zhang1,2, Baofeng Yang1,3 1Department of Pharmacology, The State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education, College of Pharmacy, Harbin Medical University, 2Institute of Metabolic Disease, Heilongjiang Academy of Medical Science, 3Department of Pharmacology and Therapeutics, Melbourne School of Biomedical Sciences, Faculty of Medicine, Dentistry and Health Sciences University of Melbourne Here we describe a new method of detecting successful establishment of shared blood circulation of two parabionts through a caudal vein injection of glucose, which causes minimal damage and is not fatal to the parabionts.