Shanghai University of Traditional Chinese Medicine 15 articles published in JoVE Medicine Gavage Strategy for Decoction Formula of Traditional Chinese Medicine in Osteosarcoma Model Mice Chujie Zhou1,2, Xingyuan Sun1,2, Junli Chang1,2, Junjie Tong1,2, Peng Zhao1,2, Suxia Guo1,2, Yanping Yang1,2 1Longhua Hospital, Shanghai University of Traditional Chinese Medicine, 2Key Laboratory of Theory and Therapy of Muscles and Bones, Ministry of Education The protocol provides a detailed standardization process of decoction formula and gavage technique with Yiqi Jiedu decoction in the osteosarcoma mouse model as an example. It describes animal protection and improves reliability of research data, providing effective strategies for investigating therapeutic efficacy and molecular mechanisms of decoction formulas in vivo. Medicine Tuina Intervention in Sodium Monoiodoacetate Injection-Induced Rat Model of Knee Osteoarthritis Yifei Xu*1, Xuanying Zhu*2, Xinrong Li*3, Yuxiang Lu*4, Jiangwei Wu3, Weimin Cai3, Juanjuan Zheng3, Zhiwei Wu5, Guangxin Guo3,6 1Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, 2Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, 3School of Acupuncture-Moxibustion and Tuina, Shanghai University of Traditional Chinese Medicine, 4School of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, 5Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, 6Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine This protocol describes the methods of Tuina intervention in sodium monoiodoacetate injection-induced rat model of knee osteoarthritis (KOA), which provides a reference for the application of Tuina in KOA animal models. This protocol also studies the effective mechanism of Tuina for KOA, and the results will help promote its application. Medicine Clinical Efficacy of Small Needle Knife Therapy on Stage I-II Frozen Shoulder Suxia Guo1, Dan Liu1, Yanping Yang1,2, Zhijun Hu1 1Longhua Hospital, Shanghai University of Traditional Chinese Medicine, 2Key Laboratory of theory and therapy of muscles and bones, Ministry of Education The protocol presented here describes the detailed operation method and proves the effectiveness of a small needle knife in the treatment of frozen shoulders. Cancer Research RNA Fluorescence In Situ Hybridization for Long Non-Coding RNA Localization in Human Osteosarcoma Cells Junli Chang*1,2, Xiaoping Ma*1,2, Xingyuan Sun1,2, Chujie Zhou1,2, Peng Zhao1,2, Yongjun Wang1,2, Yanping Yang1,2 1Longhua Hospital, Shanghai University of Traditional Chinese Medicine, 2Key Laboratory of Theory and Therapy of Muscles and Bones, Ministry of Education The present protocol describes a method of RNA fluorescence in situ hybridization to localize the lncRNAs in human osteosarcoma cells. Cancer Research Intra-Cardiac Injection of Human Prostate Cancer Cells to Create a Bone Metastasis Xenograft Mouse Model Junli Chang*1,2, Xingyuan Sun*1,2, Xiaoping Ma1,2, Peng Zhao1,2, Binhao Shi1,2, Yongjun Wang1,2, Xianghui Han1,3, Yanping Yang1,2 1Longhua Hospital, Shanghai University of Traditional Chinese Medicine, 2Key Laboratory of Theory and Therapy of Muscles and Bones, Ministry of Education, 3Institute of Chinese Traditional Surgery, Longhua Hospital, Shanghai University of Traditional Chinese Medicine Here, we present a protocol for the intra-cardiac injection of human prostate cancer cells to generate a mouse model with bone metastasis lesions. Medicine Three-Dimensional Finger Motion Tracking during Needling: A Solution for the Kinematic Analysis of Acupuncture Manipulation Liu-Liu Xu1, Fan Wang1, Hua-Yuan Yang1, Wen-Chao Tang1 1School of Acupuncture-Moxibustion and Tuina, Shanghai University of Traditional Chinese Medicine This experimental method describes a solution for the kinematic analysis of acupuncture manipulation with three-dimensional finger motion tracking technology. Cancer Research Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models Junli Chang*1,2, Fulai Zhao*1,2, Xingyuan Sun1,2, Xiaoping Ma1,2, Wenlan Zhi1,2, Yanping Yang1,2 1Longhua Hospital, Shanghai University of Traditional Chinese Medicine, 2Key Laboratory of theory and therapy of muscles and bones, Ministry of Education The present protocol describes intratibia osteosarcoma cell injection to generate mouse models bearing orthotopic osteosarcoma and pulmonary metastasis lesions. Medicine Preparation of Naringenin Solution for In Vivo Application Shufen Liu1, Jingcheng Dong2, Qin Bian2 1Spine Research Institute, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, 2Department of Integrative Medicine, Huashan Hospital, Fudan University Here, the protocol presents the preparation of naringenin solution for in vivo intraperitoneal administration. Naringenin is fully dissolved in a mixture of dimethylsulfoxide, Tween 80, and saline. The antidiabetic osteoporotic effects of naringenin were assessed by blood glucose testing, tartrate-resistant acid phosphatase staining, and enzyme-linked immunosorbent assay. Biology A Mouse Model of Lumbar Spine Instability Shufen Liu1, Yueli Sun1, Jingcheng Dong2, Qin Bian2 1Spine Research Institute, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, 2Department of Integrative Medicine, Huashan Hospital, Fudan University We developed a lumbar intervertebral disc degeneration mouse model by resection of L3–L5 spinous processes along with supra- and inter-spinous ligaments and detachment of paraspinous muscles. Medicine Acupuncture in a Rat Model of Asthma Dong-Dong Zhou1, Guang-Quan Zhang1, Qing-Yi Zhao1, Mi Cheng1, Jin Lu1, Yu Wang1, Yu-Dong Xu1, Yan-Jiao Chen1, Yong-Qing Yang1, Lei-Miao Yin1,2 1Laboratory of Molecular Biology, Shanghai Research Institute of Acupuncture and Meridian, Shanghai University of Traditional Chinese Medicine, 2Shanghai Innovation Center of Traditional Chinese Medicine Health Service A high platform can fix rats without restriction and completely expose the acupoints on the back during acupuncture manipulation. This article describes methods for the fabrication of the high platform, establishes a rat model of asthma and measures changes in respiratory function using a noninvasive and real-time whole-body plethysmography (WBP) system. Behavior Home-Based Prescribed Pulmonary Exercise in Patients with Stable Chronic Obstructive Pulmonary Disease Xiaodan Liu1,2, Peijun Li3, Jian Li3, Lu Xiao1, Ning Li3, Yufan Lu3, Zhengrong Wang3, Jianqing Su3, Zhenwei Wang4, Chunlei Shan1,2, Weibing Wu3 1School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, 2Institute of Rehabilitation Medicine, Shanghai Academy of Traditional Chinese Medicine, 3Department of Sports Medicine, Shanghai University of Sport, 4Department of Respiratory Medicine, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine Presented here is a protocol to investigate the effects of home-based prescribed pulmonary exercise in stable chronic obstructive pulmonary disease (COPD) patients, which is modified based on traditional Chinese exercises according to dyspnea and limited exercise capacity observed in COPD patients. Medicine Preparation Of Gushukang (GSK) Granules for In Vivo and In Vitro Experiments Yongjian Zhao1,2,3, Qiang Wang1,2,3, Shufen Liu1,2,3, Yongjun Wang1,2,3,4, Bing Shu1,2,3, Dongfeng Zhao1,2,3 1Longhua Hospital, Shanghai University of Traditional Chinese Medicine, 2Spine Institute, Shanghai University of Traditional Chinese Medicine, 3Key Laboratory of Theory and Therapy of Muscles and Bones, Ministry of Education, 4School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine This article provides a detailed protocol for preparing a working solution of Gushukang granules for animal studies and GSK granule containing serum for in vitro experiments. This protocol can be applied to pharmacological investigations of herbal medicines as well as prescriptions for both in vivo and in vitro experiments. Developmental Biology In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye Meng Li*1,2, Ming Hao*1,2, Dong Jiang3, Yanxi Chen4, Wen Wang1,2 1Cellular Biomedicine Group, China, 2Cellular Biomedicine Group, California, 3Department of Orthopedics, Shanghai TCM-Integrated Hospital, 4Department of Orthopedics, Shanghai East Hospital, Tongji University This protocol describes an efficient way to monitor the cell persistence and biodistribution of human adipose-derived mesenchymal stem cells (haMSCs) by far-red fluorescence labeling in a rat knee osteoarthritis (KOA) model via intra-articular (IA) injection. Medicine Preparation of Herbal Medicine: Er-Xian Decoction and Er-Xian-containing Serum for In Vivo and In Vitro Experiments Shufen Liu1, Yueli Sun1, Ji Li1, Jingcheng Dong2, Qin Bian1,2 1Longhua Hospital, Shanghai University of Traditional Chinese Medicine, 2The Academy of Integrative Medicine of Fudan University Here, we present the preparation of an er-xian decoction (EXD) in four steps—soaking, decoction, filtration, and concentration—and demonstrate the administration of a prepared EXD-containing serum to rats. These methods are applicable to the in vivo and in vitro study of herbal decoctions such as traditional Chinese medicines. Biology Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists Hao Li*1, Wei Dou*2, Emil Padikkala1, Sridhar Mani1 1Department of Genetics, Albert Einstein College of Medicine, 2Shanghai Key Laboratory of Complex Prescription and MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine Ketoconazole binds to and antagonizes Pregnane X Receptor (PXR) activation. Yeast high throughput screens of PXR mutants define a unique region for ketoconazole binding. This yeast-based genetic method discovers novel nuclear receptor interactions with ligands that associate with surface binding sites.
