JOVE JOURNAL

Biologia do Desenvolvimento

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Journal (Developmental Biology)

Visualizing Low-Abundance Proteins and Post-Translational Modifications in Living Drosophila Embryos via Fluorescent Antibody Injection

Qihong Zheng*¹, Xiaoxiang Xiang*¹, Yan Yan³, Huapeng H. Yu

¹School of Life Sciences,SUSTech University, ²Shenzhen PKU-HKUST Medical Center, ³Division of Life Science,Hong Kong University of Science and Technology

Journal (Developmental Biology)

Injecting Fluorescently Labeled Antibodies in Live Drosophila Embryos and Imaging of Low Abundance Proteins

Qihong Zheng*¹, Xiaoxiang Xiang*¹, Yan Yan³, Huapeng H. Yu

¹School of Life Sciences,SUSTech University, ²Shenzhen PKU-HKUST Medical Center, ³Division of Life Science,Hong Kong University of Science and Technology

Journal (Developmental Biology)

Generating Neural Retina from Human Pluripotent Stem Cells

Wen Li*^1,2, Hongyu Li*³, Hao Yan, Lixiong Gao, Xiaoling Wang, Lingping Zhao, Yuhan Yan², Zi Ye, Jiafei Xi, Wen Yue, Zhaohui Li²

¹Medical School of Chinese PLA, ²Senior Department of Ophthalmology,The Third Medical Center of Chinese PLA General Hospital, ³Stem Cell and Regenerative Medicine Lab,Beijing Institute of Radiation Medicine

Journal (Developmental Biology)

Culturing H9-Embryonic Stem Cells for Generation of Human Neural Retina

Wen Li*^1,2, Hongyu Li*³, Hao Yan, Lixiong Gao, Xiaoling Wang, Lingping Zhao, Yuhan Yan², Zi Ye, Jiafei Xi, Wen Yue, Zhaohui Li²

¹Medical School of Chinese PLA, ²Senior Department of Ophthalmology,The Third Medical Center of Chinese PLA General Hospital, ³Stem Cell and Regenerative Medicine Lab,Beijing Institute of Radiation Medicine

Journal (Developmental Biology)

Inducing 3D Neural Retina Formation from Cultured H9-Embryonic Stem Cells for Stem-Cell Based Therapies

Wen Li*^1,2, Hongyu Li*³, Hao Yan, Lixiong Gao, Xiaoling Wang, Lingping Zhao, Yuhan Yan², Zi Ye, Jiafei Xi, Wen Yue, Zhaohui Li²

¹Medical School of Chinese PLA, ²Senior Department of Ophthalmology,The Third Medical Center of Chinese PLA General Hospital, ³Stem Cell and Regenerative Medicine Lab,Beijing Institute of Radiation Medicine

Journal (Developmental Biology)

Preparation of Mouse Pluripotent Stem Cells (mPSCs) for Transfection

Kieran Maheden, Karen Hwang, Ipek Egilmez, Nika Shakiba

¹School of Biomedical Engineering,University of British Columbia

Journal (Developmental Biology)

An Optimized Mouse Embryonic Stem Cell Based Reverse Poly-Transfection Technique for Rapid Exploration of Nucleic Acid Ratios

Kieran Maheden, Karen Hwang, Ipek Egilmez, Nika Shakiba

¹School of Biomedical Engineering,University of British Columbia

Journal (Developmental Biology)

Reverse and Forward Transfection of Mouse Pluripotent Stem Cells (mPSCs)

Kieran Maheden, Karen Hwang, Ipek Egilmez, Nika Shakiba

¹School of Biomedical Engineering,University of British Columbia

Journal (Developmental Biology)

Flow Cytometry Analysis of Transfected Mouse Pluripotent Stem Cells (mPSCs)

Kieran Maheden, Karen Hwang, Ipek Egilmez, Nika Shakiba

¹School of Biomedical Engineering,University of British Columbia

Journal (Developmental Biology)

Experimental Preparation and Ex Ovo Culture of Chicken Embryo for Brillouin Microscopy

Chenjun Shi, Chenchen Handler, Haden Florn, Jitao Zhang

¹Department of Biomedical Engineering, College of Engineering,Wayne State University, ²Fischell Department of Bioengineering,University of Maryland

Journal (Developmental Biology)

Monitoring the Mechanical Evolution of Tissue During Neural Tube Closure of Chick Embryo

Chenjun Shi, Chenchen Handler, Haden Florn, Jitao Zhang

¹Department of Biomedical Engineering, College of Engineering,Wayne State University, ²Fischell Department of Bioengineering,University of Maryland

Journal (Developmental Biology)

Obtaining Wildtype and Transgenic Zebrafish Larvae and WildType Whole Larvae Dissociation

Naomi J. M. Kakiailatu, Laura E. Kuil², Eric Bindels, Joke T. M. Zink, Michael Vermeulen, Veerle Melotte, Maria M. Alves

¹Department of Clinical Genetics,Erasmus University Medical Center - Sophia Children's Hospital, ²Division of Psychosocial Research and Epidemiology,the Netherlands Cancer Institute, ³Department of Hematology,Erasmus Medical Center, ⁴Department of Pathology, GROW-School for Oncology and Developmental Biology,Maastricht University Medical Center

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