JOVE JOURNAL

Bioquímica

Showing 481 - 492 in 731 videos.

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Journal (Biochemistry)

Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation

Beáta Bozóki*^1,2, János András Mótyán*¹, Márió Miczi, Lívia Diána Gazda, József Tőzsér

¹Department of Biochemistry and Molecular Biology, Faculty of Medicine,University of Debrecen, ²Biotechnology Research Department,Gedeon Richter Plc

Journal (Biochemistry)

Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K

Hanan B. Elajaili, Laura Hernandez-Lagunas, Kalina Ranguelova, Sergey Dikalov, Eva Nozik-Grayck

¹Cardiovascular Pulmonary Research Laboratories and Pediatric Critical Care Medicine, Department of Pediatrics,University of Colorado Anschutz Medical Campus, ²Bruker BioSpin Corp, ³Department of Medicine, Division of Clinical Pharmacology,Vanderbilt University Medical Center

Journal (Biochemistry)

A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation

Javier A. Jaimes*^1,2, Jean K. Millet*^1,3, Monty E. Goldstein⁴, Gary R. Whittaker, Marco R. Straus

¹Department of Microbiology and Immunology, College of Veterinary Medicine,Cornell University, ²Department of Microbiology, College of Agricultural and Life Sciences,Cornell University, ³Virologie et Immunologie Moléculaires, Domaine de Vilvert,INRA, ⁴Department of Cell Biology and Molecular Genetics,University of Maryland

Journal (Biochemistry)

Identification of Functional Protein Regions Through Chimeric Protein Construction

Juan M. Adrian-Segarra, Holger Lörchner², Thomas Braun², Jochen Pöling²

¹Department of Cardiac Development and Remodelling,Max Planck Institute for Heart and Lung Research, ²Partner site Rhein-Main,German Centre for Cardiovascular Research (DZHK)

Journal (Biochemistry)

Measuring and Interpreting Oxygen Consumption Rates in Whole Fly Head Segments

Louisa Jutta Dietz, Anuroop Venkateswaran Venkatasubramani, Annika Müller-Eigner, Martin Hrabe de Angelis^4,5, Axel Imhof, Lore Becker, Shahaf Peleg^6,7

¹Munich Center of Integrated Protein Science and Biomedical Center,Ludwig-Maximilians University of Munich, ²Laboratory for Metabolism and Epigenetics in Aging,Leibniz Institute for Farm Animal Biology (FBN), ³German Mouse Clinic, Helmholtz Zentrum Munich,German Research Center for Environment and Health (GmbH), ⁴German Center for Diabetes Research (DZD), ⁵Chair of Experimental Genetics, School of Life Science Weihenstephan,Technische Universität München, ⁶Laboratory for Metabolism and Epigenetics in Brain Aging,Institute of Neuroregeneration & Neurorehabilitation of Qingdao University, ⁷Molecular Biology Division, Biomedical Center, Faculty of Medicine,Ludwig-Maximilians University of Munich

Journal (Biochemistry)

Measurement of Ion Concentration in the Unstirred Boundary Layer with Open Patch-Clamp Pipette: Implications in Control of Ion Channels by Fluid Flow

Jae Gon Kim*¹, Sang Woong Park*², Kyung Chul Shin, Bokyung Kim, Doyoung Byun, Young Min Bae

¹Department of Physiology, KU Open Innovation Center, Research Institute of Medical Science,Konkuk University School of Medicine, ²Department of Emergency Medical Services,Eulji University, ³Department of Mechanical Engineering,Sungkyunkwan University

Journal (Biochemistry)

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Bushra Husain, Sairupa Paduchuri, Sree R. Ramani, Nadia Martinez-Martin

¹Receptor Discovery group, Microchemistry, Proteomics and Lipidomics Department,Genentech, ²Portfolio Management and Operations,Genentech

Journal (Biochemistry)

Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment

Magali Court², Marie Malier², Arnaud Millet^2,3

¹Team Mechanobiology, Immunity and Cancer, Institute for Advanced Biosciences, INSERM U1209, CNRS UMR5309, ²Université Grenoble Alpes, ³Pôle Recherche,Centre Hospitalier Universitaire des Alpes

Journal (Biochemistry)

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis

Andreas Weber, Julia Christin Wehmeyer, Vera Schmidt, Artur Lichtenberg, Payam Akhyari

¹Department of Cardiovascular Surgery, Medical Faculty,Heinrich-Heine-University

Journal (Biochemistry)

How to Quantify the Fraction of Photoactivated Fluorescent Proteins in Bulk and in Live Cells

Vanessa Chen, Malte Renz

¹Gynecologic Oncology Division,Stanford University School of Medicine

Journal (Biochemistry)

Extraction and Analysis of Taiwanese Green Propolis

Chun-Ting Chen, Yi-Hsuan Chien, Yu-Hsiang Yu, Yue-Wen Chen

¹Department of Biotechnology and Animal Science,National Ilan University

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