Journal
Imunologia e Infecção
Sleeping Beauty Approach to Obtain CAR-NK Cells for Targeting Fungal Pathogens

Sleeping Beauty Approach to Obtain CAR-NK Cells for Targeting Fungal Pathogens

October 04, 2024

Author Spotlight: Developing Cellular Therapies for Invasive Fungal Infections

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1Medizinische Klinik und Poliklinik II und Lehrstuhl für Zelluläre Immuntherapie, Medizinische Klinik und Poliklinik II,Universitätsklinikum Würzburg, 2Fraunhofer-Institut für Zelltherapie und Immunologie IZI,Außenstelle Würzburg Zelluläre Immuntherapie

Summary

We outline a transfection protocol for producing chimeric antigen receptor-natural killer (CAR-NK) cells targeting fungal pathogens using the non-viral Sleeping Beauty transposon system. To assess antigen-specific activation, we co-cultured the engineered cells with Aspergillus fumigatus germ tubes and measured IFN-γ secretion.

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