Improving Student Outcomes with an Adaptable Molecular Cloning Course-Based Undergraduate Research Experience

Christopher B. Cummings; Samuel S. Catania; Eirene M. Q. Ednacot; Austin J. Kinsella-Johnson; Claire E. Meeds; Jack W. Reynolds; Ava E. Sanderson; Rachel A. Johnson; Katharine R. Watts

doi:10.3791/67067

Journal

Bioquímica

Implementing Gibson Assembly to Enhance Molecular Biology Education and Skills

November 15, 2024

Author Spotlight: Enhancing Molecular Cloning Education with Hands-On Research and Gibson Assembly

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Christopher B. Cummings*^1,2, Samuel S. Catania*^1,2, Eirene M. Q. Ednacot^1,2, Austin J. Kinsella-Johnson^1,2, Claire E. Meeds^1,2, Jack W. Reynolds^1,2, Ava E. Sanderson^1,2, Rachel A. Johnson^1,2, Katharine R. Watts^1,2

¹Department of Chemistry and Biochemistry,California Polytechnic State University, ²Center for Applications in Biotechnology,California Polytechnic State University

Summary

An adaptable Gibson Assembly molecular cloning module was employed in a course-based undergraduate research experience (CURE) format for molecular biology laboratory course students. Assessment of student learning outcomes showed improved understanding and confidence in molecular cloning after completion of the CURE, and novel plasmids were cloned for natural product biosynthesis research.

Implementing Gibson Assembly to Enhance Molecular Biology Education and Skills

Author Spotlight: Enhancing Molecular Cloning Education with Hands-On Research and Gibson Assembly

Summary

Article