Retroviral Transduction of Guide RNA for Gene Editing in Th17 Differentiation
1Shanghai Immune Therapy Institute,Renji Hospital, Shanghai Jiao Tong University School of Medicine, 2Zhejiang University-University of Edinburgh Institute (ZJU-UoE Institute),Zhejiang University School of Medicine, Zhejiang University, 3Institute of Immunology and Department of Rheumatology,Sir Run Run Shaw Hospital, Zhejiang University School of Medicine
Summary
We present a protocol for retroviral transduction of guide RNA into primary T cells from Cas9 transgenic mice, providing an efficient alternative for gene editing in studying Th17 differentiation.
