High-Throughput Screening Assay for Mycobacterium abscessus Using Dual Reporters
1i3S – Instituto de Investigação e Inovação em Saúde da Universidade do Porto, 2ICBAS – Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, 3IBMC – Instituto de Biologia Molecular e Celular, Universidade do Porto, 4Programa Doutoral em Biologia Molecular e Celular (MCBiology),Instituto de Ciências Biomédicas Abel Salazar da Universidade do Porto, 5Laboratory for Microbiology,Parasitology and Hygiene (LMPH), 6Department of Infectious Diseases in Humans, Immune Response,Sciensano
Summary
This study developed an assay for high-throughput screening against Mycobacterium abscessus with a recently created double reporter strain, using fluorescence and luminescence to assess bacterial viability quickly. This protocol will be relevant for researchers aiming to screen new drugs against this drug-resistant bacteria.
