Formation of Human Thymus Organoids in Three-Dimensional Fibrin Hydrogels

Manon d'Arco; Nathan Provin; Pierre Maminirina; Olivier Baron; Carole Guillonneau; Laurent David; Matthieu Giraud

doi:10.3791/66795

Journal

Biologia do Desenvolvimento

Recreating the Thymic Microenvironment with iPSC-Derived Organoids

October 04, 2024

Author Spotlight: Advancing Thymic Epithelial Cells and T-Cell Research with Human Thymic Organoids

01:31 min

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3D Human Thymic Organoid Model Preparation for Studying T-Cell Biology

02:04 min

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Manon d’Arco*¹, Nathan Provin*¹, Pierre Maminirina², Olivier Baron², Carole Guillonneau¹, Laurent David^1,3, Matthieu Giraud¹

¹Nantes Université, CHU Nantes, INSERM, Center for Research in Transplantation and Translational Immunology, UMR 1064, ²CHU Nantes, Nantes Université, Department of Pediatric and Congenital Cardiac Surgery, ³Nantes Université, CHU Nantes, INSERM, CNRS, BioCore, SFR Bonamy

Summary

Here, we describe a protocol for the formation of human iPSC-derived thymus organoids grown in 3D fibrin hydrogels aiming to support thymic epithelial cell (TEC) maturation and prolonged maintenance, as well as thymopoiesis in vitro.

Recreating the Thymic Microenvironment with iPSC-Derived Organoids

Author Spotlight: Advancing Thymic Epithelial Cells and T-Cell Research with Human Thymic Organoids

3D Human Thymic Organoid Model Preparation for Studying T-Cell Biology

Summary

Article