Regulating Schwann Cell Growth by Nanosecond Pulsed Electric Field for Peripheral Nerve Regeneration In Vitro

Jiahang Han; Zewei Wang; Yanzhao Dong; Xiaodi Zou; Haoyu Wang; Yonggang Chen; Sahar Ahmed Abdalbary; Tian Tu; Hui Lu

doi:10.3791/66097

Enhancing Peripheral Nerve Regeneration with nsPEF-Stimulated Schwann Cells

May 03, 2024

Regulering van Schwann-celgroei door nanoseconde gepulseerd elektrisch veld voor perifere zenuwregeneratie in vitro

01:30 min

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Preparing Cryopreserved RSC96 Cells for Nanosecond Pulsed Electric Field Stimulation

02:10 min

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Operation of the Nanosecond Pulsed Electric Field (nsPEF) Device to Stimulate Cultured RSC96 Cells

00:47 min

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Validating the Cellular Proliferation and Migration of Nanosecond Pulsed Electric Field (nsPEF) Stimulated RSC96 Cells

02:09 min

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Detectie van de expressie van de neurotrofe en transcriptiefactoren in door nanoseconden gepulseerd elektrisch veld (nsPEF) gestimuleerde RSC96-cellen

02:55 min

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Jiahang Han*^1,2, Zewei Wang*^1,2, Yanzhao Dong*¹, Xiaodi Zou, Haoyu Wang², Yonggang Chen, Sahar Ahmed Abdalbary, Tian Tu, Hui Lu

¹Department of Orthopedics, The First Affiliated Hospital, College of Medicine,Zhejiang University, ²College of Medicine,Zhejiang University, ³Department of Orthopedics,The Second Affiliated Hospital of Zhejiang Chinese Medical University, ⁴Department of Orthopedic Physical Therapy, Faculty of Physical Therapy,Nahda University in Beni Suef, ⁵Plastic & Cosmetic Center, The First Affiliated Hospital, College of Medicine,Zhejiang University

Summary

Hier presenteren we een protocol voor het toepassen van nanoseconde pulse electric field (nsPEF) om Schwann-cellen in vitro te stimuleren. De synthese en secretiecapaciteit van relevante factoren en veranderingen in celgedrag valideerden de succesvolle stimulatie met behulp van nsPEF. De studie geeft een positief beeld van de perifere zenuwregeneratiemethode.