Electronic von Frey associated with the Mouse Grimace Scale to Assess Allodynia and Pain in <em>Trypanosoma evansi</em>-Infected Mice

Daniel S&#233;rgio Cipriani; Gabriela Kaiser Borges; Luiz Claudio Miletti; Amanda Leite Bastos-Pereira

doi:10.3791/65743

Assessing Pain and Allodynia in T. evansi-Infected Animals

July 19, 2024

Author Spotlight: Assessing Pain and Promoting Animal Welfare in Laboratory Animals Infected with Trypanosoma evansi

01:36 min

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Preparation and Inoculation of Trypanosoma evansi in Mouse to Evaluate Allodynia and Pain

00:37 min

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Electronic Von Frey (EvF) Apparatus Set up for the Evaluation of Allodynia and Pain in Trypanosoma evansi Infected Animals

02:02 min

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Mechanical Threshold Assessment of Mice Infected with Trypanosoma evansi

00:49 min

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Grimace Scale Assessment of Mice Infected with Trypanosoma evansi

01:53 min

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Daniel Sérgio Cipriani, Gabriela Kaiser Borges, Luiz Claudio Miletti, Amanda Leite Bastos-Pereira³

¹Residency Program in Veterinary Medicine,Santa Catarina State University, ²Multicenter Postgraduate Program in Biochemistry and Molecular Biology,Santa Catarina State University, ³Federal University of Santa Catarina

Summary

Here, we present a protocol to evaluate nociception in mice infected with Trypanosoma evansi, using the electronic von Frey apparatus as a tool, measuring mechanical thresholds of the hind paw and viscera.