Journal
Neurociência
Rat "Brain Milking" for Neural Stem and Oligodendrocyte Progenitor Cell Isolation

Rat "Brain Milking" for Neural Stem and Oligodendrocyte Progenitor Cell Isolation

February 09, 2024

Author Spotlight: Collecting Neural Stem and Progenitor Cells from Live Animals Using a Novel Brain Milking Protocol

01:42 min
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1,220 Views

Releasing Murine Neural Stem Cells by Stereotaxic Intracerebroventricular Injection of Release Cocktail

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Collecting Murine Neural Stem and Oligodendrocyte Progenitor Cells by Cerebrospinal Fluid Liquid Biopsy

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1Laboratory of Developmental Biology, Department of Biology,University of Patras, 2Wellcome Trust-MRC Cambridge Stem Cell Institute,University of Cambridge, 3Altos Labs,Cambridge Institute of Science

Summary

A method for the isolation of neural stem cells and oligodendrocyte progenitor cells from the brains of live rats is presented here in experimental detail. It allows multiple collections of these cells from the same animals without compromising their well-being.

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