A Lightweight Drive Implant for Chronic Tetrode Recordings in Juvenile Mice

Robert J. Pendry; Lilyana D. Quigley; Lenora J. Volk; Brad E. Pfeiffer

doi:10.3791/65228

Journal

Neurociência

Micro-Drive Implant for Recording Daily Neural Activity in Juvenile Mice

June 02, 2023

Author Spotlight: A Lightweight Drive Implant for Chronic Tetrode Recordings in Juvenile Mice

01:55 min

•

939 Views

Micro-Drive Design and Construction

03:25 min

•

153 Views

Surgical Implantation of the Micro-Drive on the Mouse Skull

00:57 min

•

324 Views

Recovery after Micro-Drive Implantation

01:00 min

•

164 Views

Robert J. Pendry^1,2, Lilyana D. Quigley^1,2, Lenora J. Volk^1,3,4, Brad E. Pfeiffer^1,3

¹Department of Neuroscience,UT Southwestern Medical Center, ²Neuroscience Graduate Program,UT Southwestern Medical Center, ³O’Donnell Brain Institute,UT Southwestern Medical Center, ⁴Department of Psychiatry,UT Southwestern Medical Center

Summary

Here, we describe a micro-drive design, surgical implantation procedure, and post-surgery recovery strategy that allow for chronic field and single-unit recordings from multiple brain regions simultaneously in juvenile and adolescent mice across a critical developmental window from postnatal day 20 (p20) to postnatal day 60 (p60) and beyond.