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Chapters
- 00:05Introduction
- 00:33Surgical Robot Hardware Setup and Software Preparation
- 02:00Surgery and Skull Preparation
- 03:17Evans Blue Tail Vein Injection
- 03:57Surgical Robot Drilling Procedure
- 06:33Thermocouple Evaluation
- 07:31Results: Semi-Automated Bone Drilling of Cranial Windows to Mitigate Thermal Blood-Brain Barrier Damage
- 09:00Conclusion
Cranial windows have become a ubiquitously implemented surgical technique to allow for intravital imaging in transgenic mice. This protocol describes the use of a surgical robot that performs semi-automated bone drilling of cranial windows and can help reduce surgeon-to-surgeon variability and partially mitigate thermal blood-brain barrier damage.
