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Quantification of Vascular Parameters in Whole Mount Retinas of Mice with Non-Proliferative and Proliferative Retinopathies
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March 12, 2022
DOI:
1Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas,Universidad Nacional de Córdoba, 2Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI),Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), 3Vitreo-retinal Department,Centro Privado de Ojos Romagosa-Fundación VER, 4Unidad de Investigación y Desarrollo en Tecnología Farmacéutica (UNITEFA),Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
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This article describes a well-established and reproducible lectin stain assay for the whole mount retinal preparations and the protocols required for the quantitative measurement of vascular parameters frequently altered in proliferative and non-proliferative retinopathies.
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Vascular ParametersWhole Mount RetinasNon-proliferative RetinopathyProliferative RetinopathyOxygen-induced RetinopathyMetabolic SyndromeVascular AlterationsAvascular AreasNeovascular AreasArteriole DilatationArteriole TortuosityVascular DensityVascular BranchingRetinal DissectionImmunohistochemistryIsolectin Staining
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Subirada, P. V., Paz, M. C., Vaglienti, M. V., Luna, J. D., Barcelona, P. F., Sánchez, M. C. Quantification of Vascular Parameters in Whole Mount Retinas of Mice with Non-Proliferative and Proliferative Retinopathies. J. Vis. Exp. (181), e63126, doi:10.3791/63126 (2022).
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