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Complementation of Splicing Activity by a Galectin-3 – U1 snRNP Complex on Beads
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December 09, 2020
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This article describes the experimental procedures for (a) depletion of U1 snRNP from nuclear extracts, with concomitant loss of splicing activity; and (b) reconstitution of splicing activity in the U1-depleted extract by galectin-3 - U1 snRNP particles bound to beads covalently coupled with anti-galectin-3 antibodies.
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Voss, P. G., Haudek, K. C., Patterson, R. J., Wang, J. L. Complementation of Splicing Activity by a Galectin-3 - U1 snRNP Complex on Beads. J. Vis. Exp. (166), e61990, doi:10.3791/61990 (2020).
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