Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain

Fernando Gonz&#225;lez Ibanez; Katherine Picard; Maude Bordeleau; Kaushik Sharma; Kanchan Bisht; Marie-&#200;ve Tremblay

doi:10.3791/60510

/

Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain

JoVE 신문
면역학 및 감염병학

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE 신문 면역학 및 감염병학

Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain

29,283 Views

•

10:40 min

•

October 27, 2019

DOI:

10.3791/60510-v

DOI:

10.3791/60510-v

•

10:40 min

October 27, 2019

•

119 Views

Fernando González Ibanez^1,2, Katherine Picard^1,2, Maude Bordeleau^1,3, Kaushik Sharma^1,2,4, Kanchan Bisht^1,2,4, Marie-Ève Tremblay^1,2

¹Axe Neurosciences,Centre de Recherche du CHU de Québec-Université Laval, ²Département de Médecine Moléculaire, Faculté de Médecine,Université Laval, ³Integrated Program in Neuroscience,McGill University, ⁴Center for Brain Immunology and Glia (BIG),University of Virginia

Summary

This protocol describes a step-by-step workflow for immunofluorescent costaining of IBA1 and TMEM119, in addition to analysis of microglial density, distribution, and morphology, as well as peripheral myeloid cell infiltration in mouse brain tissue.

Explore More Videos

Immunofluorescence IBA1 TMEM119 Microglia Peripheral Myeloid Cell Density Morphology Infiltration Mouse Brain Fiji ImageJ Nearest Neighbor Distance Scale Composite Image Channel Tool Freehand Selection Selection Brush Region Of Interest

Read Article

Immunofluorescence Staining Using IBA1 and TMEM119 for Microglial Density, Morphology and Peripheral Myeloid Cell Infiltration Analysis in Mouse Brain

Summary

Automatically generated

Explore More Videos