JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Quantification of Coenzyme A in Cells and Tissues
8,037 Views
•
08:51 min
•
September 27, 2019
DOI:
Summary
Automatically generated
This method describes sample preparation from cultured cells and animal tissues, extraction and derivatization of coenzyme A in the samples, followed by high pressure liquid chromatography for purification and quantification of the derivatized coenzyme A by absorbance or fluorescence detection.
Read Article
Cite this Article
Frank, M. W., Subramanian, C., Rock, C. O., Jackowski, S. Quantification of Coenzyme A in Cells and Tissues. J. Vis. Exp. (151), e60182, doi:10.3791/60182 (2019).
Copy