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A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation
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생화학
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JoVE 신문 생화학
A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation

9,545 Views

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07:12 min

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April 24, 2020

DOI:

10.3791/59842-v

DOI:
10.3791/59842-v

•

07:12 min
April 24, 2020

•

1 Views
, , , , ,
1Advanced Clinical Biosystems Institute, Smidt Heart institute,Cedars Sinai Medical Center, 2Beckman Coulter Life Sciences

Summary

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Here, we present an automated plasma protein digestion method for mass spectrometry-based quantitative proteomic analysis. In this protocol, the liquid transferring and incubation steps for protein denaturation, reduction, alkylation, and trypsin digestion reactions are streamlined and automated. It takes approximately five hours to prepare a 96-well plate with desired precision.

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Automated WorkflowProtein Enzymatic DigestionReproducibilityThroughputBiomarker DiscoveryValidationClinical ApplicationMass SpectrometryHigh Throughput Sample PreparationQuantitative Proteomic AnalysisPlasma SampleLiquid HandlerAutomated Proteomic Sample PreparationHigh Content

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Cite this Article

Fu, Q., Johnson, C. W., Wijayawardena, B. K., Kowalski, M. P., Kheradmand, M., Van Eyk, J. E. A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation. J. Vis. Exp. (158), e59842, doi:10.3791/59842 (2020).

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