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Renal Subcapsular Transplantation of 2′-Deoxyguanosine-Treated Murine Embryonic Thymus in Nude Mice

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JoVE 신문 면역학 및 감염병학

Renal Subcapsular Transplantation of 2′-Deoxyguanosine-Treated Murine Embryonic Thymus in Nude Mice

Renal Subcapsular Transplantation of 2′-Deoxyguanosine-Treated Murine Embryonic Thymus in Nude Mice

10,317 Views

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07:39 min

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July 19, 2019

DOI:

10.3791/59657-v

DOI:

10.3791/59657-v

•

07:39 min

July 19, 2019

•

2 Views

Jianxue Wang^1,3,4, Guolin Chen^1,3,4, Qianwen Cui^2,3,4, Erfei Song^1,3,4, Weihua Tao¹, Wanqun Chen², Cunchuan Wang^1,3,4, Shiqi Jia^1,3,4

¹The First Affiliated Hospital,Jinan University, ²Department of Biochemistry and Molecular Biology, School of Basic Medical Science,Jinan University, ³The Institute of Clinical Medicine,Jinan University, ⁴Joint institute of Metabolic Medicine Between Jinan University and University of Hong Kong

Summary

We provide a simple and efficient method to transplant 2’-deoxyguanosine treated E18.5 thymus into the renal capsule of a nude mouse. This method should aide in the study of both thymic epithelial cells function and T cells maturation.

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Renal Subcapsular Transplantation Murine Embryonic Thymus Nude Mice Thymus Transplantation Thymus Epithelial Cell Function T-cell Maturation 18.5 Thymus Transplantation 2′-deoxyguanosine Treatment Thymic Transplantation

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