Journal
/
생화학
/
Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR
JoVE 신문
생화학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생화학
Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

7,299 Views

•

09:37 min

•

February 12, 2019

DOI:

10.3791/59152-v

DOI:
10.3791/59152-v

•

09:37 min
February 12, 2019

•

8 Views
, , , , ,
1Department of Chemistry,Louisiana State University, 2National High Magnetic Field Laboratory, 3Departments of Pediatrics, and Microbiology, Immunology and Parasitology,Louisiana State University Health Sciences Center

Summary

Automatically generated

A protocol for preparing 13C,15N-labeled fungal and plant samples for multidimensional solid-state NMR spectroscopy and dynamic nuclear polarization (DNP) investigations is presented.

Explore More Videos

Fungal MaterialsPlant MaterialsSolid-state NMRDynamic Nuclear PolarizationBiomaterialsCarbohydratesFungal Cell WallsCell CultureYPD MediumLabeled Minimal MediumIsotope LabelingCarbon-13Nitrogen-15SterilizationAutoclaveCell Culture Techniques

Read Article

Cite this Article

Kirui, A., Dickwella Widanage, M. C., Mentink-Vigier, F., Wang, P., Kang, X., Wang, T. Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR. J. Vis. Exp. (144), e59152, doi:10.3791/59152 (2019).

Download .ris file

Copy

Share Video

.

Copy