JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Fluorescent Visualization of Mango-tagged RNA in Polyacrylamide Gels via a Poststaining Method
8,338 Views
•
06:06 min
•
June 21, 2019
DOI:
Summary
Automatically generated
Here we present a sensitive, rapid, and discriminating post-gel staining method to image RNAs tagged with RNA Mango aptamers I, II, III, or IV, using either native or denaturing polyacrylamide gel electrophoresis (PAGE) gels. After running standard PAGE gels, Mango-tagged RNA can be easily stained with TO1-Biotin and then analyzed using commonly available fluorescence readers.
Read Article
Cite this Article
Yaseen, I. M., Ang, Q. R., Unrau, P. J. Fluorescent Visualization of Mango-tagged RNA in Polyacrylamide Gels via a Poststaining Method. J. Vis. Exp. (148), e59112, doi:10.3791/59112 (2019).
Copy