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Chapters
- 00:05Title
- 00:50Treatment of Cells, Transfection, and Seeding onto Coverslips
- 02:54Assembly of Microscopy Imaging Chamber
- 03:43Microinjection Procedure
- 05:02FRAP Procedure
- 05:32Photoactivation Procedure
- 06:26Data Analysis
- 09:42Results: Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators
- 11:27Conclusion
We describe how micro- and photomanipulation techniques such as FRAP and photoactivation enable the determination of motility parameters and the spatiotemporal dynamics of proteins within migrating cells. Experimental readouts include subcellular dynamics and turnover of motility regulators or of the underlying actin cytoskeleton.
