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ניצול מתודולוגיות משולב להגדיר את התפקיד של משלוח ממברנה פלזמה במהלך אקסון מסעף ו העצבית המורפוגנזה
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Utilizing Combined Methodologies to Define the Role of Plasma Membrane Delivery During Axon Branching and Neuronal Morphogenesis
DOI:

14:28 min

March 16, 2016

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Chapters

  • 00:05Title
  • 00:48SNARE Complex Formation Assay
  • 03:12Imaging Exocytic Events via TIRF Microscopy
  • 08:08Differential Interference Contrast Timelapse Microscopy of Axon Branching
  • 10:10Toxin Manipulations and Fixed Cell Immunofluorescence
  • 11:13Results: SNARE-mediated Exocytosis in Netrin-dependent Axon Branching
  • 12:58Conclusion

Summary

자동 번역

Light microscopy techniques coupled with biochemical assays elucidate the involvement of SNARE-mediated exocytosis in netrin-dependent axon branching. This combination of techniques permits identification of molecular mechanisms controlling axon branching and cell shape change.

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