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Super-Resolution Imaging der natürlichen Killerzell Immunologische Synapse auf einem Glas-gestützten Planar Lipiddoppelschicht
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Super-resolution Imaging of the Natural Killer Cell Immunological Synapse on a Glass-supported Planar Lipid Bilayer
DOI:

09:56 min

February 11, 2015

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Chapters

  • 00:05Title
  • 01:52Assembling the Glass-supported Planar Lipid Bilayer
  • 06:17Imaging of the NK Synapse on Lipid Bilayer Using Stimulated Emission Depletion (STED)
  • 08:11Results: Super-resolution Images of NK Cell Synapse on the Lipid Bilayer Using STED Microscope
  • 09:21Conclusion

Summary

자동 번역

We describe here a combination of the glass-supported lipid bilayer technique of forming immunological synapses with the super-resolution imaging technique of stimulated emission depletion (STED) microscopy. The goal of this protocol is to provide users with the instructions necessary to successfully carry out these two techniques.

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