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Investigating Single Molecule Adhesion by Atomic Force Spectroscopy
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Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

10,145 Views

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09:48 min

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February 27, 2015

DOI:

10.3791/52456-v

DOI:
10.3791/52456-v

•

09:48 min
February 27, 2015

•

16 Views
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1Physik-Department E22a,Technische Universität München, 2IMETUM,Technische Universität München

Summary

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A protocol to couple a large variety of single molecules covalently onto an AFM tip is presented. Procedures and examples to determine the adhesion force and free energy of these molecules on solid supports and bio-interfaces are provided.

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Stetter, F. W. S., Kienle, S., Krysiak, S., Hugel, T. Investigating Single Molecule Adhesion by Atomic Force Spectroscopy. J. Vis. Exp. (96), e52456, doi:10.3791/52456 (2015).

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