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Isolation of Distinct Cell Populations from the Developing Cerebellum by Microdissection
Chapters
- 00:05Title
- 01:09General Preparations
- 02:41Dissection and Preparation of Tissue Slices
- 04:57Tissue Microdissection and Cell Dissociation, Sorting, and Plating
- 06:46Results of Isolating and Culturing NEPs
- 07:43Conclusion
Nestin-expressing progenitors are a newly identified population of neuronal progenitors in the developing cerebellum. Using the microdissection technique presented here in combination with fluorescent-activated cell sorting, this cell population can be purified with no contamination from other cerebellar regions and can be cultured for further studies.
