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A Flow Adhesion Assay to Study Leucocyte Recruitment to Human Hepatic Sinusoidal Endothelium Under Conditions of Shear Stress
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Chapters
- 00:05Title
- 01:59Cytokine Stimulation of Endothelial Cells
- 04:11Isolation and Pretreatment of Peripheral Blood Lymphocytes
- 06:09Setup of Flow Assay System
- 08:33Flow AssayTechnique and Recording of Adhesion for Analysis
- 09:35Results: Adhesion Cascade of Blood Lymphocytes over Hepatic Sinusoidal Endothelium
- 10:44Conclusion
Leucocyte recruitment to the liver occurs within the specialized channels of the hepatic sinusoids which are lined by unique hepatic sinusoidal endothelial cells. Phase contrast microscopy of leucocyte recruitment across human hepatic sinusoidal endothelium under conditions of physiological shear stress can facilitate the elucidation of the molecular mechanisms which underlie this process.
Tags
Keywords:
Flow Adhesion AssayLeucocyte RecruitmentHuman Hepatic Sinusoidal EndotheliumShear StressInflammatory Liver DiseasesFibrosisCirrhosisMolecular MechanismsTherapeutic TargetsLeucocyte InfiltrationHepatic SinusoidsHepatic Sinusoidal Endothelial Cells (HSEC)Adhesion CascadeTetheringActivationFirm AdhesionCrawlingTransmigrationPhase Contrast MicroscopyInhibitors
