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Forebrain Electrophysiological Recording in Larval Zebrafish
Chapters
- 00:05Title
- 00:46Egg Production and Collection
- 01:30Microelectrode Pulling and Preparation
- 02:02Immobilizing Zebrafish for Recordings
- 03:14Extracellular Field Recording
- 05:00Electrographic Seizure-like Discharge Recordings
- 05:45Conclusion
A simple method to record extracellular field potentials in the larval zebrafish forebrain is described. The method provides a robust in vivo read-out of seizure-like activity. This technique can be used with genetically modified zebrafish larvae carrying epilepsy-related genes or seizures evoked by administration of convulsant drugs.
