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Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI
Chapters
- 00:05Title
- 01:52Assemble and Calibrate the Ultrasound System
- 03:03Animal Preparation
- 05:12Blood-brain Barrier Opening with Microbubbles and UltraSound (BOMUS) and Neuronal Stimulation
- 06:28Magnetic Resonance Imaging and Analysis
- 06:58Results: Functional Neuroimaging using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI
- 07:54Conclusion
A technique is described for broadly opening the blood-brain barrier in the mouse using microbubbles and ultrasound. Using this technique, manganese can be administered to the mouse brain. Because manganese is an MRI contrast agent that accumulates in depolarized neurons, this approach enables imaging of neuronal activity.
Tags
Functional NeuroimagingUltrasonic Blood-brain Barrier DisruptionManganese-enhanced MRIMiceGenetic And Molecular Underpinnings Of NeuroscienceAIM MRINeuronal Activity MappingMn2+Calcium AnalogMRI EnhancementMn2+ ClearanceBlood-brain Barrier (BBB)UltrasoundGas-filled MicrobubblesUltrasound Contrast AgentsBBB Opening MethodPerflutren Microbubbles
