JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
कुल शाही सेना अलगाव और डाउनस्ट्रीम अगली पीढ़ी के अनुक्रमण और जीन एक्सप्रेशन प्रोफाइलिंग के लिए कैद गैर अलहदा माउस ब्रेन क्षेत्र के Microdissection के लिए लेजर माइक्रोस्कोपी दृष्टिकोण
Chapters
- 00:05Title
- 01:40Sampling Setup and Preparation
- 04:30Brain Removal and Coronal Sectioning
- 06:05Brain Region Microdissection and Tissue Archival
- 07:44Total RNA Extraction
- 09:01Representative RNA Isolation Results
- 09:38Conclusion
RNA expression profiling of discrete mouse brain regions requires a precise and repeatable tissue collection strategy. A protocol that uses both coronal brain sectioning and tissue corer-assisted microdissection is described here. The yield and quality of total RNA obtained from the resulting samples confirms the utility of the outlined method.
Tags
Non-laser Capture MicroscopyMicrodissectionMouse Brain RegionsTotal RNA IsolationNext-generation SequencingGene Expression ProfilingTechnological PlatformsNext-generation SequencingMicroarrayQRT-PCRHigh-resolution RNA SequencingRNA-SeqTranscript LevelsAlternative SplicingMicro RNAsWhole Transcriptome ProfilingPhenotypically Distinct GroupsMolecular MechanismsPhysiological StatesDisease ProgressionNext-generation Sequencing IntegrationGene Expression StudiesMouse ModelsBrain Nuclei PrecisionLaser Capture Microscopy (LCM)Sample Excision
