Ikke-Laser Capture Mikroskopi Tilgang til mikrodissektion af diskrete Mouse Brain Regioner for Total RNA Isolation og downstream næste generation sekventering og Gene Expression Profiling
Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling
Ikke-Laser Capture Mikroskopi Tilgang til mikrodissektion af diskrete Mouse Brain Regioner for Total RNA Isolation og downstream næste generation sekventering og Gene Expression Profiling
RNA expression profiling of discrete mouse brain regions requires a precise and repeatable tissue collection strategy. A protocol that uses both coronal brain sectioning and tissue corer-assisted microdissection is described here. The yield and quality of total RNA obtained from the resulting samples confirms the utility of the outlined method.