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Targeting Olfactory Bulb Neurons Using Combined In Vivo Electroporation and Gal4-Based Enhancer Trap Zebrafish Lines
Chapters
- 00:05Title
- 01:07Transgenic Embryos
- 01:55Mounting Embryos
- 03:37Micro-injection of DNA Expression Plasmid
- 04:55Electroporation
- 06:00Mounting Embryos for Imaging
- 07:03Expression Targeted to Developing Olfactory Bulb
- 07:55Conclusion
The temporal and spatial resolution of genetic manipulations determines the spectrum of biological phenomena that they can perturb. Here we use temporally and spatially discrete in vivo electroporation, combined with transgenic lines of zebrafish, to induce expression of a GFP transgene specifically in neurons of the developing olfactory bulb.
Tags
Olfactory Bulb NeuronsIn Vivo ElectroporationGal4-based Enhancer TrapZebrafish LinesDNA Expression PlasmidsRNAi ReagentsMorpholino Anti-sense OligonucleotidesDeveloping EmbryosC. ElegansChickXenopusMouseSpatial ResolutionTemporal ResolutionTransgenic ApproachesSpecific Cell PopulationDeveloping Nervous System
