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染色质免疫沉淀从以下轴索损伤的背根神经节组织
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Chromatin Immunoprecipitation from Dorsal Root Ganglia Tissue following Axonal Injury
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染色质免疫沉淀从以下轴索损伤的背根神经节组织

DOI:
10.3791/2803-v

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09:41 min

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July 20, 2011

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, ,
1Laboratory for NeuroRegeneration and Repair, Department of Neurology, Hertie Institute for Clinical Brain Research,University of Tuebingen , 2Graduate School for Cellular and Molecular Neuroscience,University of Tuebingen

Chapters

  • 00:05Title
  • 01:04Sciatic & Dorsal Column Nerve Injury
  • 04:35Cross-linking
  • 06:14Immunoprecipitation and Elution
  • 08:23Increased Expression After Nerve Injury
  • 09:11Conclusion

Summary

자동 번역

자동 번역

我们目前从背根神经节组织中的染色质免疫沉淀性轴索损伤的方法。这种方法可以用来识别特定的转录因子结合位点，同时在周边及中枢神经系统受伤的轴突再生的表观遗传修饰的组蛋白与DNA的重要。

Tags

Chromatin ImmunoprecipitationDorsal Root GangliaAxonal InjuryCentral Nervous SystemPeripheral Nervous SystemNeurite OutgrowthAxonal RegenerationTranscriptional ProgramsGene RegulationInjury Model SystemTranscriptional ActivityAnimal RemovalNerve FibersSciatic NerveSpinal CordTranscription Factor BindingHistone ModificationDNA ModificationChIP Protocol

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