Cellular Impedance-Based Survival Kinetics Assay to Assess Virus Survival
Published: April 30, 2023
Abstract
Source: Labadie, T. et al., Monitoring Influenza Virus Survival Outside the Host Using Real-Time Cell Analysis. J. Vis. Exp. (2021).
This video describes a cellular impedance-based technique to study the survival kinetics of viruses that induce cytopathic effects. The method measures the electrical impedance of virus-infected adherent cells pre-treated with saline.
Protocol
1. Preparation of reagents and starting materials Preparation of MDCK cells and sterile cell culture medium Cultivate Madin-Darby Canine Kidney (MDCK) cells in modified Eagle's medium (MEM) supplemented with 10% heat-inactivated fetal calf serum (FCS) and antibiotics (100 units/mL penicillin, 100 mg/mL streptomycin). After thawing, passage MDCK cells at least 2x before infecting them to ensure complete recovery (but less than 30 passages to avoid any drift in cell phenotypes). …
Disclosures
The authors have nothing to disclose.
Materials
| 0.25% Trypsin | ThermoFisher | 25200056 | |
| 75 cm2 tissue culture flask | Falcon | 430641U | |
| E-Plate 16 (6 plates) | ACEA Biosciences, Inc | 5469830001 | E-plates are available in different packaging |
| FCS | Life technologies (gibco) | 10270-106 | |
| MEM 1X | Life technologies (gibco) | 31095029 | |
| PBS 1X | Life technologies (gibco) | 14040091 | |
| Penicillin-Streptomycin | Life technologies (gibco) | 11548876 | |
| TPCK-Trypsin | Worthington | LS003740 | |
| xCELLigence Real-Time Cell Analysis Instrument S16 | ACEA Biosciences, Inc | 380601310 | The xCELLigence RTCA S16 instruments are available in different formats (16-well, 96-well, single or multi-plate |
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Cite This Article
Cellular Impedance-Based Survival Kinetics Assay to Assess Virus Survival. J. Vis. Exp. (Pending Publication), e21325, doi: (2023).