Metastasi sperimentale e CTL adottivi Immunoterapia modello di trasferimento del mouse
Summary
Una metastasi al polmone sperimentale e CTL modello murino immunoterapia per l'analisi delle cellule tumorali interazione cellule-T<em> In vivo</em>.
Abstract
Sperimentale modello murino metastasi è un modello metastasi semplice ma fisiologicamente rilevanti. Le cellule tumorali vengono iniettate per via endovenosa (iv) nelle vene coda di topo e colonizzare nei polmoni, quindi, che assomiglia le ultime fasi della metastasi delle cellule tumorali spontanea: la sopravvivenza nel stravaso circolazione, e la colonizzazione negli organi distali. Da un punto di vista terapeutico, il modello sperimentale metastasi è il modello più semplice e ideale, poiché l'obiettivo delle terapie è spesso il punto finale di metastasi: stabilito tumore metastatico nell'organo distale. In questo modello, le cellule tumorali vengono iniettate nelle vene iv coda di topo e ha permesso di colonizzare e crescere nei polmoni. CTL tumore-specifici sono poi iniettate iv in metastasi portante del mouse. Il numero e le dimensioni delle metastasi polmonari possono essere controllati in base al numero delle cellule tumorali da iniettare e il momento della crescita tumorale. Pertanto, le varie fasi di metastasi, da metastasi minimo metastasi estese, possono essere modellati. Metastasi polmonari vengono analizzati con l'inflazione con l'inchiostro, permettendo così più facile l'osservazione visuale e la quantificazione.
Protocol
Disclosures
The authors have nothing to disclose.
Acknowledgements
Supportato anche da finanziamenti del National Institutes of Health (CA133085 a KL) e l'American Cancer Society (RSG-09-209-01-TBG a KL).
Materials
Solutions:
India Ink Solution (17):
- Pour 150 ml of distilled water into a 250 ml flask.
- Add 4 drops ammonium hydroxide to the distilled water.
- Add 30 ml India Ink stock (i.e. Sanford Black Magic Waterproof Drawing Ink 4465 Item 44011) to the ammonia and water mixture.
- Top off with distilled water to a volume of 200 ml. Solution is ready for injection.
Fekete’s Solution (17):
Fekete’s solution is used to bleach India ink-inflated tumor-bearing lungs to distinguish white tumor nodules from the black background of normal tissues.
- Add 350ml 95% EtOH to 1L glass bottle.
- Add 150ml distilled water
- Add 50ml formaldehyde
- Add 25ml glacial acidic acid
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