如何建立一个激光散斑对比度成像（LSCI）系统，以监测血流
Summary
本视频演示了如何构建一个激光散斑对比度成像（LSCI）系统，可以很容易地被用来监测血液流量。
Abstract
激光散斑对比度成像（LSCI）是一个简单而强大的技术，是满场血流显像。技术分析在动态散斑检测如何激光多普勒频移进行分析来确定粒子的速度运动的粒子的波动。因为它可以用来监测的红血细胞的运动，LSCI已成为一个流行的工具测量组织如视网膜，皮肤和大脑的血流量。在神经科学，其中可量化的生理活动过程中，如功能激活，中风和蔓延的去极化的血流量的变化，它已成为特别有用。 LSCI也有吸引力，因为它提供了良好的空间和时间分辨率，而使用廉价的仪器，可以很容易地与其他成像方式相结合。在这里，我们展示如何构建一个LSCI设置和证明其有能力，监测在动物实验中大脑中的血液流量的变化。
Protocol
Discussion
在这段视频中，我们已经证明建立和使用激光散斑对比成像（LSCI）系统血流量的变化看，它是多么容易。 LSCI在20世纪80年代开发的一种方式产生1视网膜中的血液流动的地图。虽然仍用于图像的视网膜和皮肤灌注，它已成为极受欢迎，作为一个图像在大脑中 2血流技术。这主要是由于出色的空间和时间分辨率和简单的仪器。 LSCI已被用来调查血流量的变化，由于3,4功能激活?…
Disclosures
The authors have nothing to disclose.
Acknowledgements
作者承认由美国心脏协会（0735136N），达纳基金会，美国国家科学基金会（CBET/0737731），库尔特基金会的支持。
Materials
| Material Name | Type | Company | Catalogue Number | Comment |
|---|---|---|---|---|
| Firewire camera | Basler | scA640-74f | ||
| Macro zoom lens | Edmund Optics | NT58-240 | ||
| Laser diode | Thorlabs | HL6501MG | ||
| Laser diode controller | Thorlabs | LDC201CU |
The technique is versatile enough to be used with a wide range of equipment. The only things necessary to perform the experiment are a compatible camera with a lens, a laser diode of any type with a controller, and the provided software. A table of the specific equipment used in the video is included above.
A complete list of additional parts that can be used in this experiment is found on our website, http://bach.bme.utexas.edu/mediawiki/index.php/Hardware
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