JOVE JOURNAL

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Showing 457 - 468 in 3,291 videos.

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Journal (Biology)

Optimized Production and Analysis of Recombinant Protein-Filled Vesicles from E. coli

Bree R. Streather¹, Karen Baker¹, Tara A. Eastwood¹, Daniel P. Mulvihill¹

¹School of Biosciences,University of Kent

Journal (Biology)

Recombinant Vescicle Isolation from Bacterial Culture to Release Soluble Protein

Bree R. Streather¹, Karen Baker¹, Tara A. Eastwood¹, Daniel P. Mulvihill¹

¹School of Biosciences,University of Kent

Journal (Biology)

Visualizing Recombinant Protein-Filled Vesicles from E. coli by Fluorescence Microscopy

Bree R. Streather¹, Karen Baker¹, Tara A. Eastwood¹, Daniel P. Mulvihill¹

¹School of Biosciences,University of Kent

Journal (Biology)

An Adapted Optical Density-Based Microplate Assay for Characterizing Actinobacteriophage Infection

Elijah I. Christenson¹, Qingyang Zhang², Ruth Plymale¹

¹Department of Biology,Ouachita Baptist University, ²Department of Mathematical Sciences,University of Arkansas

Journal (Biology)

Microplate Preparation for Actinobacteriophage Infection

Elijah I. Christenson¹, Qingyang Zhang², Ruth Plymale¹

¹Department of Biology,Ouachita Baptist University, ²Department of Mathematical Sciences,University of Arkansas

Journal (Biology)

Actinobacteriophage Infection and Absorbance Measurement

Elijah I. Christenson¹, Qingyang Zhang², Ruth Plymale¹

¹Department of Biology,Ouachita Baptist University, ²Department of Mathematical Sciences,University of Arkansas

Journal (Biology)

Luciferase Assay for the Detection of Autophagy Protease ATG4B

Denise R. B. Pilger¹, Christin Luft¹, Robin Ketteler^1,2

¹Laboratory for Molecular Cell Biology,University College London, ²Department of Human Medicine,Medical School Berlin

Journal (Biology)

Image Acquisition and Analysis for the Detection of Autophagy Protease ATG4B Using Luciferase Assay

Denise R. B. Pilger¹, Christin Luft¹, Robin Ketteler^1,2

¹Laboratory for Molecular Cell Biology,University College London, ²Department of Human Medicine,Medical School Berlin

Journal (Biology)

Cell-Based Drug Screening for Inhibitors of Autophagy Related 4B Cysteine Peptidase

Denise R. B. Pilger¹, Christin Luft¹, Robin Ketteler^1,2

¹Laboratory for Molecular Cell Biology,University College London, ²Department of Human Medicine,Medical School Berlin

Journal (Biology)

Autophagy Protease Tag-Compound Addition and Cellular Supernatant Harvest for Drug Screening

Denise R. B. Pilger¹, Christin Luft¹, Robin Ketteler^1,2

¹Laboratory for Molecular Cell Biology,University College London, ²Department of Human Medicine,Medical School Berlin

Journal (Biology)

Transfection, Isolation, and Screening of the CENP-E Knockout HeLa Cells

Meng-Fei Xu^1,2, Jie Chen^1,2, Yue Xu^1,2, Jing-Lian Zhang^1,2, Yi Zhou^1,2, Jie-Jie He^1,2, Shan Wu^1,2, Ya-Lan Wei^3,4, Zhen-Yu She^1,2

¹Department of Cell Biology and Genetics, The School of Basic Medical Sciences,Fujian Medical University, ²Key Laboratory of Stem Cell Engineering and Regenerative Medicine,Fujian Province University, ³College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics,Fujian Medical University, ⁴Medical Research Center,Fujian Maternity and Child Health Hospital

Journal (Biology)

Immunofluorescence Staining and High-Resolution Confocal Microscopy of CRISPR/Cas9-Mediated CENP-E Knockout HeLa Cells

Meng-Fei Xu^1,2, Jie Chen^1,2, Yue Xu^1,2, Jing-Lian Zhang^1,2, Yi Zhou^1,2, Jie-Jie He^1,2, Shan Wu^1,2, Ya-Lan Wei^3,4, Zhen-Yu She^1,2

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