In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts
Summary
Nuclear membrane assembly is an essential step in the cell division cycle; this process can be replicated in the test tube by combining Xenopus sperm chromatin, cytosol, and light membrane fractions. Complete nuclei are formed, including nuclear membranes with pore complexes, and these reconstituted nuclei are capable of normal nuclear processes.
Abstract
Nuclear membrane assembly is an essential step in the cell division cycle; this process can be replicated in the test tube by combining Xenopus sperm chromatin, cytosol, and light membrane fractions. Complete nuclei are formed, including nuclear membranes with pore complexes, and these reconstituted nuclei are capable of normal nuclear processes.
Protocol
The complete text protocol for this experimental approach is available in Current Protocols in Cellular Biology.
Disclosures
The authors have nothing to disclose.
Cite This Article
Cross, M., Powers, M. In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts. J. Vis. Exp. (19), e908, doi:10.3791/908 (2008).