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May 10, 2024
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We describe a protocol to non-invasively and continuously measure absolute microvascular blood flow index and blood oxygen saturation using a multi-modal device based on near-infrared diffuse optics. We then evaluate the metabolic rate of oxygen consumption and reactive hyperemia utilizing a vascular occlusion test.
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Yaqub, M. A., Zanoletti, M., Cortese, L., Sánchez, D. S., Amendola, C., Frabasile, L., Karadeniz, U., Garcia, J. M., Martin, M., Cortes-Picas, J., Caballer, A., Cortes, E., Nogales, S., Tosi, A., Carteano, T., Garcia, D. S., Tomanik, J., Wagenaar, T., Mui, H., Guadagno, C. N., Parsa, S., Venkata Sekar, S. K., Demarteau, L., Houtbeckers, T., Weigel, U. M., Lacerenza, M., Buttafava, M., Torricelli, A., Contini, D., Mesquida, J., Durduran, T. Non-Invasive Monitoring of Microvascular Oxygenation and Reactive Hyperemia using Hybrid, Near-Infrared Diffuse Optical Spectroscopy for Critical Care. J. Vis. Exp. (207), e66062, doi:10.3791/66062 (2024).
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