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Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma
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Neuroscience
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JoVE Journal Neuroscience
Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma

Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma

English

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1,483 Views

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10:02 min

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June 02, 2023

DOI:

10.3791/64924-v

DOI:
10.3791/64924-v

•

10:02 min
June 02, 2023

•

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1Department of Biochemistry and Molecular Biology, Faculty of Pharmacy,Universidad de Sevilla, 2Instituto de Biomedicina de Sevilla, Hospital Universitario Virgen del Rocío, CSIC,Universidad de Sevilla

Summary

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The present protocol describes the purification steps and subsequent studies of four different fungal β-glucans as potential immunomodulatory molecules that enhance the anti-tumoral properties of microglia against glioblastoma cells.

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Beta-glucanFungal PolysaccharidesImmunotherapyGlioblastomaMicrogliaMushroom ExtractsPleurotusHericiumGanodermaSoluble Mushroom PolysaccharidesInsoluble Mushroom PolysaccharidesAlpha-amylaseGlucoamylaseAlkalaseExtractionPurification

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Folgado-Dorado, C., Caracena-De La Corte, J., Aguilera-Velázquez, J. R., Santana-Villalona, R., Rivera-Ramos, A., Carbonero-Aguilar, M. P., Talaverón, R., Bautista, J., Sarmiento Soto, M. Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma. J. Vis. Exp. (196), e64924, doi:10.3791/64924 (2023).

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