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En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos
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Developmental Biology
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JoVE Journal Developmental Biology
En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos

En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos

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1,602 Views

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08:57 min

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July 27, 2022

DOI:

10.3791/64207-v

DOI:
10.3791/64207-v

•

08:57 min
July 27, 2022

•

3 Views
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1Intercellular Signalling in Cardiovascular Development and Disease Laboratory,Centro Nacional de Investigaciones Cardiovasculares (CNIC), 2Ciber de Enfermedades Cardiovasculares

Summary

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Classically, the endocardium of the mouse embryonic valve primordium has been analyzed using transversal, coronal, or sagittal sections. Our novel approach for en face, two-dimensional imaging of the endocardium at valvulogenic regions allows planar polarity and cell rearrangement analysis of the endocardium during valve development.

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Cite this Article

Gonzalez-Costa, T., de la Pompa, J. L., Grego-Bessa, J. En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos. J. Vis. Exp. (185), e64207, doi:10.3791/64207 (2022).

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