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Visualizing Subcellular Localization of a Protein in the Heart Using Quantum Dots-Mediated Immuno-Labeling Followed by Transmission Electron Microscopy
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Biology
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Visualizing Subcellular Localization of a Protein in the Heart Using Quantum Dots-Mediated Immuno-Labeling Followed by Transmission Electron Microscopy

Visualizing Subcellular Localization of a Protein in the Heart Using Quantum Dots-Mediated Immuno-Labeling Followed by Transmission Electron Microscopy

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2,421 Views

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08:13 min

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September 16, 2022

DOI:

10.3791/64085-v

DOI:
10.3791/64085-v

•

08:13 min
September 16, 2022

•

14 Views
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1Department of Pathology and Translational Pathobiology,Louisiana State University Health Sciences Center-Shreveport, 2Department of Molecular and Cellular Physiology,Louisiana State University Health Sciences Center-Shreveport

Summary

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The present protocol describes a method of immuno-labeling a protein in the heart tissue sections using quantum dots. This technique provides a useful tool to visualize any protein's subcellular localization and expression at the ultrastructural level.

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Subcellular LocalizationProteinHeartQuantum DotsImmunolabelingTransmission Electron MicroscopyFixationOsmicationContrast Enhancement

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Aishwarya, R., Abdullah, C. S., Remex, N. S., Nitu, S., Hartman, B., King, J., Bhuiyan, M. S. Visualizing Subcellular Localization of a Protein in the Heart Using Quantum Dots-Mediated Immuno-Labeling Followed by Transmission Electron Microscopy. J. Vis. Exp. (187), e64085, doi:10.3791/64085 (2022).

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