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Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures

Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures

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09:26 min

June 27, 2022

DOI:

09:26 min
June 27, 2022

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Summary

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This versatile protocol describes the isolation of premigratory neural crest cells (NCCs) through the excision of cranial neural folds from chick embryos. Upon plating and incubation, migratory NCCs emerge from neural fold explants, allowing for assessment of cell morphology and migration in a simplified 2D environment.

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